For the impatient
=================

The latest source code can be downloaded from github and compiled using:

    git clone --recurse-submodules git://github.com/samtools/htslib.git
    git clone git://github.com/samtools/bcftools.git
    cd bcftools
     # The following is optional:
     #   autoheader && autoconf && ./configure --enable-libgsl --enable-perl-filters
    make


    IMPORTANT:
    ---
    In order to use the BCFtools plugins, this environment variable must be set and point
    to the correct location

        export BCFTOOLS_PLUGINS=/path/to/bcftools/plugins

    ---

Note that if also the polysomy command or support for Perl filtering should be included,
the compilation instructions differ, see Optional Compilation with GSL and Optional
Compilation with Perl below.



System Requirements
===================

BCFtools and HTSlib depend on the following libraries:

  BCFtools:
    zlib       <http://zlib.net>
    gsl        <https://www.gnu.org/software/gsl/>
               (optional, for the 'polysomy' command)
    libperl    <http://www.perl.org/>
               (optional, to support filters using perl syntax)

  HTSlib:
    zlib       <http://zlib.net>
    libbz2     <http://bzip.org/>
    liblzma    <http://tukaani.org/xz/>
    libcurl    <https://curl.haxx.se/>
               (optional but strongly recommended, for network access)
    libcrypto  <https://www.openssl.org/>
               (optional, for Amazon S3 support; not needed on MacOS)

Building them requires development files to be installed on the build machine;
note that MacOS and some Linux distributions package these separately from the
library itself. See the "System Specific Details" below for guidance on how to
install these on a variety of systems.

The bzip2 and liblzma dependencies can be removed if full CRAM support
is not needed - see HTSlib's INSTALL file for details.

To build BCFtools, you will need:

    GNU make
    C compiler (e.g. gcc or clang)

In addition, building the configure script requires:

    autoheader
    autoconf

Running the configure script uses awk, along with a number of
standard UNIX tools (cat, cp, grep, mv, rm, sed, among others).  Almost
all installations will have these already.

Running the test harness (make test) uses:

    bash
    perl

Building Configure
==================

This step is only needed if configure.ac has been changed, or if configure
does not exist (for example, when building from a git clone).  The
configure script and config.h.in can be built by running:

    autoheader
    autoconf

If you have a full GNU autotools install, you can alternatively run:

    autoreconf

Warnings like "AC_CONFIG_SUBDIRS: you should use literals" can be ignored
or suppressed using 'autoconf -Wno-syntax'.


Compilation
===========

'cd' to the bcftools directory containing the package's source and type:

    ./configure
    make

If installing from a release (as opposed to from GitHub), the BCFtools release
tarball contains a copy of HTSlib which will be used to build BCFtools. If you
already have a system-installed HTSlib or another HTSlib that you would
prefer to build against, you can arrange this by using the configure script's
--with-htslib option. Use --with-htslib=DIR to point to an HTSlib source tree
or installation in DIR (if the desired source tree has been configured to
build in a separate build directory, DIR should refer to the build directory);
or use --with-htslib=system to ignore any nearby HTSlib source tree and use
only a system-installed HTSlib.

When --with-htslib is not used, configure looks for an HTSlib source tree
within or alongside the BCFtools source directory; if there are several
likely candidates, you will have to use --with-htslib to choose one. When
using make without running configure first, the directory ../htslib is used.


Optional Compilation with Perl
==============================

The '-i' and '-e' options can take external perl scripts for a more
sophisticated filtering. This option can be enabled by supplying the
--enable-perl-filters option to configure before running make:

    ./configure --enable-perl-filters

Note that enabling this option changes the license from MIT to GPL because bcftools
need to be built with

    perl -MExtUtils::Embed -e ccopts -e ldopts

Note that when compiling on MacOS, the default options for linking with Perl
provided by the system sometimes do not work. It was reported that removing
the occurrence of -arch i386 from config.mk solved the problem.


Optional Compilation with GSL
=============================

The 'polysomy' command depends on the GNU Scientific Library (GSL) and is not
enabled by default. In order to compile it, supply the --enable-libgsl
option to configure before running make:

    ./configure --enable-libgsl

The GNU Scientific Library depends on the cblas library.  The configure
script will look for both libcblas and libgslcblas, in that order.  If
you have more than one version of cblas installed and want to override its
choice you can do this by using either --with-cblas=cblas or
--with-cblas=gslcblas.

Note that GSL is distributed under the GNU General Public License (GPL).
When --enable-libgsl is used to compile bcftools, the resulting program
will also be GPL licensed.  If you want to distribute the program, you MUST
do so under terms compatible with that license.  A copy of the GPL is included
in the LICENSE file, or can be downloaded from
<http://www.gnu.org/copyleft/gpl.html>.

In the default compilation mode the program is dual licensed and you may
choose to be licensed under the terms of the MIT/Expat license or the
GNU General Public License (GPL).

Information on the GNU Scientific Library, including the rationale for
its choice of license can be found at <https://www.gnu.org/software/gsl/>.

Installation
============

Type 'make install' to install the bcftools executable and associated scripts
and a manual page.  The default is to install in /usr/local.  This can be
changed by using the configure script's --prefix option:

   ./configure --prefix=/path/to/install/dir

The install target also understands DESTDIR and the other usual installation
directory variables.

The bgzip and tabix utilities are provided by HTSlib.  If you have not also
installed HTSlib separately, you may wish to install these utilities by hand
by copying bcftools-1.x/htslib-1.x/{bgzip,tabix} to the same bin directory
to which you have installed bcftools et al.



System Specific Details
=======================

Installing the prerequisites is system dependent and there is more
than one correct way of satisfying these, including downloading them
from source, compiling and installing them yourself.

For people with super-user access, we provide an example set of commands
below for installing the dependencies on a variety of operating system
distributions.  Note these are not specific recommendations on distribution,
compiler or SSL implementation.  It is assumed you already have the core set
of packages for the given distribution - the lists may be incomplete if
this is not the case.

Debian / Ubuntu
---------------

sudo apt-get update  # Ensure the package list is up to date
sudo apt-get install autoconf automake make gcc perl zlib1g-dev libbz2-dev liblzma-dev libcurl4-gnutls-dev libssl-dev libperl-dev libgsl0-dev

Note: libcurl4-openssl-dev can be used as an alternative to libcurl4-gnutls-dev.

RedHat / CentOS
---------------

sudo yum install autoconf automake make gcc perl-Data-Dumper zlib-devel bzip2 bzip2-devel xz-devel curl-devel openssl-devel gsl-devel perl-ExtUtils-Embed

Alpine Linux
------------

Note: To install gsl-dev, it may be necessary to enable the "community"
repository in /etc/apk/repositories.

sudo apk update  # Ensure the package list is up to date
sudo apk add autoconf automake make gcc musl-dev perl bash zlib-dev bzip2-dev xz-dev curl-dev libressl-dev gsl-dev perl-dev

OpenSUSE
--------

sudo zypper install autoconf automake make gcc perl zlib-devel libbz2-devel xz-devel libcurl-devel libopenssl-devel gsl-devel


MacOS, assuming Xcode is installed:
-----
xz
gsl (optional)


